| MMP-1

Interstitial collagenase, also known as fibroblast collagenase and matrix metalloproteinase-1 (MMP-1), is an enzyme that in humans is encoded by the MMP1 gene.The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.[2] MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA.MMP-1 has an estimated molecular mass of 54 kDa.MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin-like domain.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	600	300	150	75	37.5	18.75	9.37	0
OD	2.56	1.84	1.22	0.81	0.47	0.26	0.15	0.07
Corrected OD	2.49	1.77	1.15	0.74	0.4	0.19	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Monkey MMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-102	97
Plasma(n=8)	94-107	103
Cell Culture Supernates(n=8)	96-110	106

 

| Linearity

Samples were spiked with high concentrations of Monkey MMP-1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-93	88-96	90-110
	Average (%)	90	92	97
1 : 4	Range (%)	89-103	87-110	105-115
	Average (%)	94	98	109